A new tool to study genetic expression using 2-D electrophoresis data: the functional map concept

Thierry Rabilloud (1) , Pierre Vincens (2) , Philippe Tarroux (3)

1 LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux
2 IBENS - Institut de biologie de l'ENS Paris
3 LIMSI - Laboratoire d'Informatique pour la Mécanique et les Sciences de l'Ingénieur

A method derived from general computerized data analysis techniques is used here first to tabulate the characteristics of individual peptide spots observed by two-dimensional electrophoresis and to compare these characteristics among spots. Multivariate analysis of such data arrays then leads to the grouping of spots in an n-dimensional space, according to their expression characteristics. Such maps display zones which are characteristic of the tissue studied. These functional maps provide a powerful analytical tool for describing such functional interactions and also for predicting the function of unidentified peptides observed on the electrophoresis gels.

Domains: Genomics [q-bio.GN]

Thierry Rabilloud, Pierre Vincens, Philippe Tarroux. A new tool to study genetic expression using 2-D electrophoresis data: the functional map concept. FEBS Letters, 1985, 189 (2), pp.171-178. ⟨10.1016/0014-5793(85)81018-8⟩. ⟨hal-03739131⟩