Quantifying hydroxyl radicals generated by a low-temperature plasma using coumarin: methodology and precautions

Quantification par la coumarine des radicaux hydroxyle générés par des plasma basse température: méthodologie et précautions

Florent Ducrozet (1, 2) , Amal Sebastian (3) , Cecilia Julieta Garcia Villavicencio (3) , Sylwia Ptasinska (3, 4) , Cécile Sicard-Roselli (1, 2)

1 ICP - Institut de Chimie Physique
2 Université Paris-Saclay
3 UND - University of Notre Dame [Indiana]
4 Department of Physics [Notre Dame]

The detection and quantification of hydroxyl radicals (HO•) generated by low-temperature plasmas (LTPs) are crucial for understanding their role in diverse applications of plasma radiation. In this study, the formation of HO• in the irradiated aqueous phase is investigated at various plasma parameters, by probing them indirectly using the coumarin molecule. We propose a quantification methodology for these radicals, combining spectrophotometry to study the coumarin reaction with hydroxyl radicals and fluorimetry to evaluate the formation yield of the hydroxylated product, 7-hydroxycoumarin. Additionally, we thoroughly examine and discuss the impact of pH on this quantification process. This approach enhances our comprehension of HO• formation during LTP irradiation, adding valuable insights to plasma’s biological applications.

Domaines: Chimie

Florent Ducrozet, Amal Sebastian, Cecilia Julieta Garcia Villavicencio, Sylwia Ptasinska, Cécile Sicard-Roselli. Quantifying hydroxyl radicals generated by a low-temperature plasma using coumarin: methodology and precautions. Physical Chemistry Chemical Physics, 2024, 26, pp.8651 - 8657. ⟨10.1039/d4cp00040d⟩. ⟨hal-04513503⟩